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KMID : 0606920210290030273
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Biomolecules & Therapeutics
2021 Volume.29 No. 3 p.273 ~ p.281
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Differential Signaling and Virus Production in Calu-3 Cells and Vero Cells upon SARS-CoV-2 Infection
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Park Byoung-Kwon
Kim Dong-Bum
Park Sang-Kyu
Maharjan Sony
Kim Jin-Soo
Choi Jun-Kyu
Akauliya Madhav
Lee Young-Hee
Kwon Hyung-Joo
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Abstract
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Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is responsible for the current coronavirus disease 2019 (COVID-19) pandemic. Signaling pathways that are essential for virus production have potential as therapeutic targets against COVID-19. In this study, we investigated cellular responses in two cell lines, Vero and Calu-3, upon SARS-CoV-2 infection and evaluated the effects of pathway-specific inhibitors on virus production. SARS-CoV-2 infection induced dephosphorylation of STAT1 and STAT3, high virus production, and apoptosis in Vero cells. However, in Calu-3 cells, SARS-CoV-2 infection induced long-lasting phosphorylation of STAT1 and STAT3, low virus production, and no prominent apoptosis. Inhibitors that target STAT3 phosphorylation and dimerization reduced SARS-CoV-2 production in Calu-3 cells, but not in Vero cells. These results suggest a necessity to evaluate cellular consequences upon SARS-CoV-2 infection using various model cell lines to find out more appropriate cells recapitulating relevant responses to SARS-CoV-2 infection in vitro.
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KEYWORD
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Apoptosis, COVID-19, SARS-CoV-2, STAT1, STAT3, STAT3 phosphorylation
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